A centrifugal ultrafiltration strategy for isolating the low-molecular weight (≤ 25 K) component of human plasma proteome

DW Greening; RJ Simpson

Journal article

A centrifugal ultrafiltration strategy for isolating the low-molecular weight (≤ 25 K) component of human plasma proteome

DW Greening, RJ Simpson

Journal of Proteomics | Published : 2010

DOI: 10.1016/j.jprot.2009.09.013

Abstract

The low-molecular weight fraction (LMF) of the human plasma proteome is an invaluable source of biological information, especially in the context of identifying plasma-based biomarkers of disease. In this study, a separation and enrichment strategy based on centrifugal ultrafiltration was developed for the LMF (i.e., ≤ 25 K) of plasma routinely prepared from normal, healthy volunteers. Four commercially-available filter membranes of similar nominal molecular weight cut-off (NMWC), but differing membrane chemistries and filter orientations (Microcon®, Millipore; Centrisart®, Sartorius; Amicon Ultra®, Millipore; Vivaspin®, Sartorius), were evaluated. Of these filtration devices, only the Sarto..

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University of Melbourne Researchers

David Greening Author

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Grants

Awarded by Australian National Health and Medical Research Council

Awarded by NHMRC

Funding Acknowledgements

Funding was provided, in part, by the Australian National Health and Medical Research Council under Program Grant 487922 (RJS, DWG), and a University of Melbourne PostGraduate Student Scholarship (DWG). We acknowledge the NHMRC-funded Australian Proteomics Computational Facility (APCF) under Enabling Grant 381413. We wish to thank Suresh Mathivanan from the Ludwig Institute for Cancer Research for his integration and comparison of plasma proteome studies and in contributing to Fig. 3.